產(chǎn)品名稱 編號(hào) 產(chǎn)品說(shuō)明 單位 單價(jià) 加入購(gòu)物車
His-tag蛋白純化磁珠（IDA-Ni) 70501-5 5 mL, 10%(v/v), 30-150 μm 瓶 ￥580.00
His-tag蛋白純化磁珠（IDA-Ni) 70501-100 2×50 mL, 10%(v/v), 30-150 μm 盒 ￥5000.00
His-tag蛋白純化磁珠（IDA-Ni) 70501-1000 4×250mL, 10%(v/v), 30-150 μm 盒 ￥30000.00
His-tag蛋白純化試劑盒（IDA-Ni) 70501-K10 10次反應(yīng)試劑盒(5 mL) 盒 ￥580.00
His-tag蛋白純化磁珠（IDA-Co) 70502-5 5 mL, 10%(v/v), 30-150 μm 瓶 ￥580.00
His-tag蛋白純化磁珠（IDA-Co) 70502-100 2x50 mL, 10%(v/v), 30-150 μm 盒 ￥5000.00
His-tag蛋白純化磁珠（IDA-Co) 70502-1000 4x250 mL, 10%(v/v), 30-150 μm 盒 ￥30000.00
His-tag蛋白純化試劑盒（IDA-Co) 70502-K10 10次反應(yīng)試劑盒(5 mL) 盒 ￥580.00
His-tag蛋白純化磁珠（NTA-Ni) 70521P-5 5 mL, 25% (v/v), 10-30 μm 瓶 ￥980.00
His-tag蛋白純化磁珠（NTA-Ni) 70521P-10 10 mL, 25%(v/v), 10-30 μm 瓶 ￥1580.00
His-tag蛋白純化磁珠（NTA-Ni) 70521P-100 2×50 mL, 25%(v/v), 10-30 μm 瓶 ￥12640.00

BeaverBeads? His-tag Protein Purification磁珠（組氨酸標(biāo)簽蛋白純化瓊脂糖磁珠）是專為高效、快速純化組氨酸標(biāo)簽蛋白而設(shè)計(jì)的一種新型功能化材料，可通過(guò)磁性分離方式直接從生物樣品中一步純化出高純度的目標(biāo)蛋白，顯著地簡(jiǎn)化了純化工藝，降低了設(shè)備需求，適合科研和工業(yè)領(lǐng)域便捷快速地進(jìn)行組氨酸標(biāo)簽蛋白的純化工作。

對(duì)比傳統(tǒng)的過(guò)柱層析純化方式， His-tag Protein Purification磁珠通過(guò)磁性分離方式純化組氨酸標(biāo)簽蛋白，無(wú)需對(duì)粗蛋白樣品進(jìn)行多次費(fèi)時(shí)費(fèi)力的離心、過(guò)濾步驟，無(wú)需裝柱和過(guò)柱層析，無(wú)需昂貴的柱層析設(shè)備，在1小時(shí)內(nèi)便能便捷地獲得高產(chǎn)量和高純度的目的蛋白，且能輕松實(shí)現(xiàn)多樣品平行處理，顯著提高了工作效率，大大降低了設(shè)備、時(shí)間和人工成本.

本產(chǎn)品系列包括鎳離子（Nickel）和鈷離子（Cobalt）兩種金屬離子螯合磁珠。它們?cè)谀繕?biāo)蛋白結(jié)合量及所得目標(biāo)蛋白純度上有所差異，一般推薦客戶使用鎳離子螯合磁珠，大多數(shù)情況下能滿足客戶對(duì)蛋白載量和純度的要求；對(duì)純度要求更高的客戶推薦使用鈷離子螯合磁珠。

His-tag Protein Purification磁珠廣泛適用于細(xì)菌、酵母、昆蟲和哺乳動(dòng)物細(xì)胞等分泌或胞內(nèi)表達(dá)的可溶性組氨酸標(biāo)簽蛋白以及變性蛋白的純化。


產(chǎn)品特點(diǎn)

1.可從粗樣品直接純化目標(biāo)蛋白，極大的縮短純化時(shí)間

2. 可輕松實(shí)現(xiàn)對(duì)目標(biāo)蛋白濃度和體積的控制

通過(guò)調(diào)節(jié)洗脫緩沖液體積的方式，實(shí)現(xiàn)對(duì)目標(biāo)蛋白濃度和體積的控制，無(wú)蛋白損失，且不存在蛋白變性、沉淀的風(fēng)險(xiǎn)。

3. 一步純化即可獲得高純度目標(biāo)蛋白

海貍磁珠與市場(chǎng)上進(jìn)口產(chǎn)品相比，一步純化即得到同水平高純度的目標(biāo)蛋白。一個(gè)完整的操作流程即可獲得純度可以達(dá)到95％以上的目標(biāo)蛋白。

4.平行操作穩(wěn)定性高，可方便實(shí)現(xiàn)高通量和大規(guī)模蛋白純化

目的蛋白純度和產(chǎn)量的標(biāo)準(zhǔn)差均<5%。

完全不存在流速和樣品體積的限制，適用于高通量和大規(guī)模蛋白純化。

5.可以獲得較高的目標(biāo)蛋白產(chǎn)率

利用海貍磁珠對(duì)粗樣品進(jìn)行純化，一步完整的純化流程之后，90％以上純度的目標(biāo)蛋白，其產(chǎn)率一般達(dá)到70～80％。

6.可重復(fù)使用，再生工藝簡(jiǎn)單

由圖A、B可知，海貍磁珠反復(fù)進(jìn)行多達(dá)六次再生處理，仍能保持較好的目標(biāo)蛋白結(jié)合能力和純度。

圖A. 再生6次后磁珠結(jié)合目標(biāo)蛋白檢測(cè)圖譜         圖B. 再生6次后磁珠的蛋白結(jié)合量數(shù)據(jù)圖

 7. 鎳螯合磁珠與鈷螯合磁珠對(duì)不同分子量的His-tag蛋白純化對(duì)比

? Co離子螯合磁珠蛋白結(jié)合量比Ni離子螯合磁珠稍低，但純度更高；

? Co離子螯合磁珠洗滌和洗脫所用咪唑濃度都比Ni離子螯合磁珠低。

         圖A.鎳和鈷螯合磁珠純化His-tag Lif 蛋白                          圖B.鎳和鈷螯合磁珠純化M-MLv蛋白

                  （分子量～ 50KD）對(duì)比                                                    （分子量～ 70KD）對(duì)比

 

引用文獻(xiàn)：

  1. Microbiota-derived succinate promotes enterohaemorrhagic Escherichia coli virulence via lysine succinylation.nature microbiology.2025（IF=19.4)

  2. The 14-3-3 protein CaTFT7 interacts with transcription factor CaHDZ27 to positively regulate pepper immunity against Ralstonia solanacearum.Horticulture Research.2025,(IF=8.5)

  3. USP8‐Governed MDA5 Homeostasis Promotes Innate Immunity and Autoimmunity.Advanced Science.2025(IF:14.1)

  4. SMALL AND ROUND GRAIN is involved in the brassinosteroid signaling pathway which regulates grain size in rice.Journal of Integrative Plant Biology.2025(IF:9.3)

  5. Characterization of the second type of tubuliform spidroin (TuSp1 variant 2) elucidates the essential role of cysteine within the repetitive domain in liquid-liquid phase separation-mediated silk formation and the mechanical properties of silk fibers.International Journal of Biological Macromolecules.2025(IF:8.5)

  6. Characterization, immune response and antibacterial mechanism of CXCL12 in black rockfish (Sebastes schlegelii).International Journal of Biological Macromolecules.2025(if:8.5)

  7. N terminus and C terminus of Arabidopsis P4-ATPase AtALA1 facilitate the detoxification of the mycotoxin deoxynivalenol in wheat.Cell Reports.2025,(IF=6.9)

  8. Odorant-Binding Protein 33 Mediates Detection of the Host-Associated Compounds in Spodoptera litura Larvae.Journal of Agricultural and Food Chemistry.2025(IF=6.2)

  9. Identification of Adult Attractants through Functional Analyses of Chemosensory Protein 21 from the Bean Bug Riptortus pedestris.Journal of Agricultural and Food Chemistry.2025(IF=6.2)

  10. Engineering Corynebacterium glutamicum with Effective Heme Supply for the Synthesis of High-Activity Hemoglobins and Myoglobins.Journal of Agricultural and Food Chemistry..2025(IF=6.2)

  11. Screening, characterization and specific binding mechanism of aptamers against human plasminogen Kringle 5. Bioorganic Chemistry, 2023.

  12. MADS-box protein AGL8 interacts with chromatin-remodelling component SWC4 to activate thermotolerance and environment-dependent immunity in pepper. Analytica chimica acta, 2023.

  13. Structural basis for the self-recognition of sDSCAM in Chelicerata. Nature communications, 2023.

  14. RPL21 interacts with LAMP3 to promote colorectal cancer invasion and metastasis by regulating focal adhesion formation. Cellular & molecular biology letters, 2023.

  15. A novel protein elicitor (PeSy1) from Saccharothrix yanglingensis induces plant resistance and interacts with a receptor-like cytoplasmic kinase in Nicotiana benthamiana. Molecular Plant Pathology, 2023.

  16. The CsHSFA-CsJAZ6 module mediated high temperature regulates flavonoid metabolism in Camellia sinensis. Plant, cell & environment, 2023.

  17. Proteomic analysis of extracellular vesicles from tick hemolymph and uptake of extracellular vesicles by salivary glands and ovary cells. Parasites & vectors, 2023.

  18. Pseudorabies Virus Regulates the Extracellular Translocation of Annexin A2 To Promote Its Proliferation. Journal of virology, 2023.

  19. Stepwise Optimization of Inducible Expression System for the Functional Secretion of Horseradish Peroxidase in Saccharomyces cerevisiae. Journal of agricultural and food chemistry, 2023.

  20. Functional differentiation of two general-odorant binding proteins in Hyphantria cunea (Drury) (Lepidoptera: Erebidae). Pest management science, 2023.

  21. 4ENB1 encodes a cellulose synthase 5 that directs synthesis of cell wall ingrowths in maize basal endosperm transfer cells. Plant Cell 2022 03 04;34(3). 

  22. A plant immune protein enables broad antitumor response by rescuing microRNA deficiency. Cell 2022 May 26;185(11). DOI: doc88.com/p-08661512614974.html. 

  23. aSWC4 regulates the immunity-thermotolerance tradeoff by recruiting CabZIP63/CaWRKY40 to target genes and activating chromatin in pepper. PLoS Genet 2022 02;18(2). 

  24. Characterization of a novel type homoserine dehydrogenase with high oxidation activity from Arthrobacter nicotinovorans. Process Biochemistry 114 (2022) 102–110. 

  25. Heat shock protein 101 contributes to the thermotolerance of male meiosis in maize. Plant Cell. 2022 Jun 27;koac184. DOI: 10.1093/plcell/koac184/6618537?login=false.

  26. Ligand-binding properties of odorant-binding protein 6 in Athetis lepigone to sex pheromones and maize volatiles. Pest Manag Sci 2022 Jan;78(1).

  27. Mycoplasma synoviae dihydrolipoamide dehydrogenase is an immunogenic fibronectin/plasminogen binding protein and a putative adhesin. Vet Microbiol 2022 Feb;265.

  28. Small Molecules Promote Selective Denaturation and Degradation of Tubulin Heterodimers through a Low-Barrier Hydrogen Bond. Jmedchem, 2022. DOI: 10.1021/acs.jmedchem.2c00379.

  29. The Therapeutic Effect of an Anti-TNF-α/HSA/IL-6R Triple-Specific Fusion Protein Under Experimental Septic Conditions. Inflammation 2022 Apr;45(2).

  30. Type One Protein Phosphatase Regulates Fixed-Carbon Starvation-Induced Autophagy by Dephosphorylating ATG13a to Facilitate ATG1a-ATG13a Formation in Arabidopsis. bioRxiv, 2022.

  31. Enhanced Thermostability of Pseudomonas nitroreducens Isoeugenol Monooxygenase by the Combinatorial Strategy of Surface Residue Replacement and Consensus Mutagenesis.Catalysts, 2021, 11.DOI:10.3390/catal11101199.

  32. Corrigendum to: Proteomics analysis reveals the importance of transcriptional regulator slyA in regulation of several physiological functions in Aeromonas hydrophila.Journal of Proteomics, 2023, Oct, 24(250). DOI: 10.1016/j.jprot.2021.104390.

  33. Bluetongue Virus Non-structural Protein 3 (NS3) and NS4 Coordinatively Antagonize Type Ⅰ Interferon Signaling by Targeting STAT1.Veterinary Microbiology, 2021, 254:108986.DOI:10.1016/j.vetmic.2021.108986.

  34. Fluorescent assay for quantitative analysis of trimethylamine N-oxide. Analytical Methods, 2021, 13(12): 1527-1534. DOI: 10.1039/d0ay02353a.

  35. Administration of fusion cytokines induces tumor regression and systemic antitumor immunity. MedComm, 2021, 2(2): 256-268. DOI: 10.1002/mco2.68.

  36. Characterization of a Novel Type Homoserine Dehydrogenase Only with High Oxidation Activity from Arthrobacter nicotinovorans. bioRxiv, 2021. DOI: 10.1101/2021.02.09.430557.

  37. Construction and directional evolution of antienrofloxacin ScFv antibody for the immunoassay of fluoroquinolone. Research Square. DOI: 10.21203/rs.3.rs-310107/v2.

  38. SARS-COV-2 RBD Oral Vaccine Boosted by Mucosal Immune Adjuvant LTB26 via DCs and B Cells Activation in Mice. bioRxiv, 2021. doi.org/10.1101/2020.04.06.025981.

  39. The therapeutic effect of a novel anti-TNF-α/IL-6R triple-specific fusion protein under experimental septic condition. Research Square. DOI: 10.21203/rs.3.rs-298423/v1.

  40. Overexpression of a cysteine proteinase inhibitor gene from Jatropha curcas confers enhanced tolerance to salinity stress；ELECTRONIC JOURNAL OF BIOTECHNOLOGY  卷: 18   期: 5   頁(yè): 368-375   出版年: SEP 2015

  41. Production and Stabilization of an Integrin Binding Moiety of Complement Component 3；MOLECULAR BIOLOGY  卷: 49   期: 5   頁(yè): 723-727   出版年: SEP 2015

  42. The role of semaphorin 4D as a potential biomarker for antiangiogenic therapy in colorectal cancer；ONCOTARGETS AND THERAPY  卷: 9   頁(yè): 1189-1204   出版年: 2016

  43. 血紅鉚釘菇免疫調(diào)節(jié)蛋白基因克隆及其生物信息學(xué)分析和重組表達(dá)；沈陽(yáng)農(nóng)業(yè)大學(xué)學(xué)報(bào) 

  44. Innovation Spaces in Asia: Entrepreneurs, Multinational Enterprises and Policy；

  45. 登革病毒四型聯(lián)合重組包膜蛋白III 區(qū)的表達(dá)和免疫原性鑒定*；中國(guó)生物工程雜志 

  46. Quantitative Proteomic Analysis of Escherichia coli Heat-Labile Toxin B Subunit (LTB) with Enterovirus 71 (EV71) Subunit VP1；INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES  卷: 17   期: 9     文獻(xiàn)號(hào): 1419   出版年: SEP 2016

  47. 人C-Src 蛋白酪氨酸激酶真核表達(dá), 純化及活性檢測(cè)；生物技術(shù)通報(bào) 

  48. 人酪氨酸蛋白激酶Lyn的真核表達(dá)、純化及鑒定；生物技術(shù) 

  49.  一個(gè)麻風(fēng)樹Kunitz型蛋白酶抑制劑基因的克隆和鑒定；四川大學(xué)學(xué)報(bào)(自然科學(xué)版) 

  50. 鋅離子依賴金屬蛋白酶1(Zmp1)抑制小鼠RAW264.7細(xì)胞增殖以及吞噬體-溶酶體的融合；細(xì)胞與分子免疫學(xué)雜志 41. A Novel Assay for Screening Inhibitors Targeting HIV Integrase LEDGF/p75 Interaction Based on Ni2+ Coated Magnetic Agarose Beads；SCIENTIFIC REPORTS  卷: 6     文獻(xiàn)號(hào): 33477   出版年: SEP 16 201642. 42. Eukaryotic expression,protein purification and identification of human tyrosine-protein kinase Lyn；

  51. The role of semaphorin 4D as a potential biomarker for antiangiogenic therapy in colorectal cancer；ONCOTARGETS AND THERAPY  卷: 9   頁(yè): 1189-1204   出版年: 2016

  52. Design, Recombinant Fusion Expression and Biological Evaluation of Vasoactive Intestinal Peptide Analogue as Novel Antimicrobial Agent；Molecules 2017, 22, 1963; DOI:10.3390/molecules22111963

  53. The thermoduric effects of site-directed mutagenesis of proline and lysine on dextransucrase from Leuconostoc mesenteroides 0326；International Journal of Biological Macromolecules，DOI：10.1016/j.ijbiomac.2017.10.023

  54. GR1-like gene expression in Lycium chinense was regulated by cadmium-induced endogenous jasmonic acids accumulation；Plant Cell Rep (2017) 36:1457–1476, DOI:10.1007/s00299-017-2168-2

  55. Development of a peptide ELISA to discriminate vaccine-induced immunity from natural infection of hepatitis A virus in a phase IV study；Eur J Clin Microbiol Infect Dis (2017) 36:2165–2170, DOI:10.1007/s10096-017-3040-6

    暫無(wú)視頻